Nisitrol is a natural antimicrobial agent and used as a natural food preservative. The active ingredient is Nisin on a 50% salt base. Nisin is effective against wide range of Gram-positive bacteria particularly spore forming bacteria.
It inhibits certain strains of food pathogens, such as Clostridium botulinum, Bacillus subtilis, Bacillus stearothermophilus, Staphylococcus aureus and Listeria.
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Nisin was recognised as a food preservative by FAO/WHO in 1969.
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Nisin was granted GRAS status by US FDA in 1988.
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Nisin has an EU number of E234.
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Nisin has a NZFSA number as a preservative 234.
Methods of Application:
Nisin is applied in solution or as dry powder mixed into food during processing with heat. It is also applied in surface treatment by spray or immersion method or in packaging material. Nisin is most stable at pH 3.0 in solution.
Application of Nisin:
Nisin is used in many food products in the range of 3.0 to 8.0 pH.
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Food Products
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Usage Levels
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Benefit of Application
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Dairy Products:
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Processed cheese and cheese spreads
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100 - 200 mg/kg
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Extends shelf life by 4 to 6 months
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Direct acidified cheese
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100 - 200 mg/kg
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Extends shelf life by 2 to 3 months
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Fresh and recombined milk
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50 - 100 mg/kg
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Extends shelf life by 3 to 5 days
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Cream and cream products
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50 - 100 mg/kg
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Extend shelf life by 10 to 15 days
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Pasteurized dairy desserts
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100 - 200 mg/kg
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Extends shelf life by 7 to 10 days
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Canned evaporated milk
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80 - 100 mg/kg
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Reduces heating time by 8 to 10 min
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Soy milk
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80 - 100 mg/kg
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Extends shelf life of UHT milk.
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Soybean curd
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100 - 200 mg/kg
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Extends shelf life of bean curd
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Dressings and sauces
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50 -100 mg/kg
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Extends shelf life by 2 to 3 weeks
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Canned peas, beans, and potato
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100 - 150 mg/kg
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Reduces canning time
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Canned mushrooms
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150 - 200 mg/kg
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Reduces canning time
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Retort pouch packet food
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100 - 200 mg/kg
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Extends shelf life
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Fruits and vegetable juices
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50 - 100 mg/kg
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Extend shelf life by 7 to 10 days
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Bottled vegetables
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100 - 200 mg/kg
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Reduces canning time
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Liquid egg products
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150 - 200 mg/kg
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Extends shelf life
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Fresh coconut milk
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80 - 100 mg/kg
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Extends shelf life by 2 to 3 days
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Flour products like pasta, noodles
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80 - 100 mg/kg
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Extends shelf life by few days
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Muffins and cakes
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100 -150 mg/kg
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Extends shelf life by 1 to 2 weeks
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Beer and related products
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50 - 80 mg/kg
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Extends shelf life
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Meat and sausages
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200 ppm*
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* Use with 200 ppm nitrate
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